biolog phenotypic microarrays  (Biolog Inc)

Journal: Nature Communications

Article Title: Suppression of gut colonization by multidrug-resistant Escherichia coli clinical isolates through cooperative niche exclusion

doi: 10.1038/s41467-025-61327-7

Figure Lengend Snippet: A Results of competition assay in Biolog® Phenotypic microarray competitive index (CFU/ml E. coli MDR1/ CFU/ml E. coli MR102) and OD 600 of the respective strains of all carbon sources that both strains could utilize. One dot represents data from three independent experiments. B Results of competition assay in Biolog® Phenotypic microarray competitive index ( E. coli MDR1/ E. coli MR102) of all carbon sources that could be utilized by at least one of the strains. One dot represents data from three independent experiments. C Competition assay in MM9 supplemented with 5 g/L of the respective carbon source under aerobic and anaerobic conditions. P values indicate a nonparametric Kruskal-Wallis test * p < 0.05. Mean and SD with one dot representing data from one experiment performed in duplicates or triplicates. D Resulting fecal burden of E. coli MDR1 after different time points of colonization. Geometric mean and SD of two independent experiments with n = 8 (PBS), n = 9 (WT) and n = 10 ( ΔmanA and manA : :manA ) mice per group. E Commensal E. coli MR102 colonization levels at different time points. Geometric mean and SD of two independent experiments withof two independent experiments with n = 8 (PBS), n = 9 (WT) and n = 10 ( ΔmanA and manA : :manA ). F Clearance kinetics of E. coli MDR1 after different time points of colonization (clearance = CFU/g below the detection limit in feces). P -values represent the Log-rank (Mantel-Cox) test with 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: B Results of competition assay in Biolog® Phenotypic microarray competitive index ( E. coli MDR1/ E. coli MR102) of all carbon sources that could be utilized by at least one of the strains.

Techniques: Competitive Binding Assay, Microarray

Journal: mBio

Article Title: Physiological roles of an Acinetobacter -specific σ factor

doi: 10.1128/mbio.00968-25

Figure Lengend Snippet: SigAb mitigates and responds to copper stress. (A) Heatmap of heavy metal resistance genes significantly upregulated (log 2 FC > 1, FDR < 0.05, T = 10 min) in RNA-seq time course experiment of sigAb overexpression strain compared to empty vector control. Operons are denoted to the right, with arrows indicating the direction of transcription. (B) Growth curves plotted as OD 600 over time (h) of CRISPRi sigAb knockdown (KD) strain and non-targeting (NT) control in rich defined medium with 250 µg/mL CuSO 4 stress ( n = 3). Data are represented as mean ± s.d. for NT control with no stress (red), sigAb KD with no stress (blue), NT control with copper stress (green), and sigAb KD with copper stress (purple). (C) sigAb KD growth defects in metal stresses graphed as area under the curve normalized to NT control ( n = 2–9). Data are represented as the mean ± s.d., and significance was calculated with a two-tailed Student’s t -test ( P < 0.05). Bars without asterisks are not significantly different from the control. (D) SigAb induction curves plotted as P sigAb activity (mRFP fluorescence) versus cell density (OD 600 ) for metal and antibiotic stress conditions using Biolog Phenotype Microarray PM13.

Article Snippet: To expand our phenotyping to additional conditions (e.g., manganese, cobalt), we tested the growth of the sigAb knockdown in Biolog Phenotype Microarray (PM) plates ( ; ).

Techniques: RNA Sequencing, Over Expression, Plasmid Preparation, Control, Knockdown, Two Tailed Test, Activity Assay, Fluorescence, Microarray